candidate_id	variant_id	Gene	gene	Protein_change	protein_change	mutant_peptide	HLA	TPM	tumor_AO	tumor_VAF	RNA_ALT_reads	RNA_alt_reads	RNA_VAF	MT_EL_rank	WT_EL_rank	dna_support_score	expression_score	rna_allele_support_score	mhc_binding_score	mt_wt_specificity_score	safety_score	experimental_actionability_score	risk_penalty	EVN_score_default	EVN_rank_default	EVN_score_calibrated	EVN_rank_calibrated	PF_score_pre_validation	PF_rank_pre_validation	PF_score_post_validation	PF_rank_post_validation	EVN_PF_score_additive	EVN_PF_rank_additive	EVN_PF_score_reweighted	EVN_PF_rank_reweighted	primer_id	recommended_primer	has_primer_design	primer_match_method	primer_quality_score	primer_specificity_score	amplicon_suitability_score	mutation_position_score	off_target_penalty	technical_risk_penalty	evidence_tier_final	tier_label	tier_reason	tier_positive_factors	tier_limiting_factors	tier_risk_flags	evidence_completeness_score	is_final_shortlist	is_top5_wet_candidate	sanger_support_label
chr19:16093699:G:C|AQFAERTVAK|HLA-A*11:01	chr19:16093699:G:C	TPM4	TPM4	p.E204Q	p.E204Q	AQFAERTVAK	HLA-A*11:01	288.172947	115	0.5502392344497608	2738.0	2738.0	0.5803306485799068	0.102729	2.368014	0.4157594165390263	0.8547018640702909	0.7901653242899533	0.6449630945515498	0.7101984100531522	0.6944444444444444	1.0	0.0169444444444444	0.7088710936113352	4	0.6832780437064345	5	0.9733333333333334	1	1.0	1	0.9137368939466104	4	0.8385892564282731	4	TPM4_ALT_P2	TPM4_ALT_P2	1	gene_protein_change	0.8666666666666667	1.0	1.0	1.0	0.0	0.0	Tier 1	High-confidence experimentally actionable candidates	High EVN/PF priority with strong biological evidence and actionable primer feasibility. Risk flags: normal_tissue_caution.	high_evn_pf_rank;strong_mhc_binding;expressed;dna_supported;rna_allele_supported;primer_feasible;experimentally_reviewed_candidate;wet_lab_prioritized_candidate	safety_caution	normal_tissue_caution	1.0	1	1	ALT peak support
chrX:41344255:G:C|STVRPCVVY|HLA-A*29:02	chrX:41344255:G:C	DDX3X	DDX3X	p.R278T	p.R278T	STVRPCVVY	HLA-A*29:02	122.802216	181	1.0	928.0	928.0	1.0	0.271904	2.175808	0.7163530233035451	0.7086493133896047	0.9316998186733014	0.5537228461284591	0.6427261271884227	0.777618769470405	0.5	0.0156190615264797	0.6363850294783607	46	0.6199406366536503	39	0.52	1539	0.52	1539	0.7447560008372599	66	0.7190604576408417	43			0	unmatched	0.5	0.5	0.5	0.5	0.0	0.0	Tier 2	Biologically promising but experimentally less ready candidates	Biologically promising candidate requiring additional experimental design or evidence completion. Risk flags: near_self_caution;self_match_penalty.	high_evn_pf_rank;strong_mhc_binding;expressed;dna_supported;rna_allele_supported;acceptable_safety_profile;experimentally_reviewed_candidate	limited_primer_feasibility	near_self_caution;self_match_penalty	1.0	1	0	
chr3:172353067:C:T|SETTYRIRIQA|HLA-B*45:01	chr3:172353067:C:T	FNDC3B	FNDC3B	p.P927S	p.P927S	SETTYRIRIQA	HLA-B*45:01	75.513664	66	0.2578125	154.0	154.0	0.2516339869281045	0.322035	4.133623	0.1915660857765364	0.6258018241633304	0.4444021655291233	0.5378612956875165	0.6728618334490288	0.9638142523364486	0.5	0.0023092873831775	0.547370004387816	812	0.6154669051876787	45	0.52	1541	0.52	1541	0.7377994351971875	77	0.7130473746949436	49			0	unmatched	0.5	0.5	0.5	0.5	0.0	0.0	Tier 2	Biologically promising but experimentally less ready candidates	Biologically promising candidate requiring additional experimental design or evidence completion.	high_evn_pf_rank;strong_mhc_binding;expressed;dna_supported;rna_allele_supported;acceptable_safety_profile;experimentally_reviewed_candidate	limited_primer_feasibility		1.0	1	0	
chr12:889170:C:G|AELRRTLSP|HLA-B*45:01	chr12:889170:C:G	WNK1	WNK1	p.Q1552E	p.Q1552E	AELRRTLSP	HLA-B*45:01	66.68063699999999	209	0.6572327044025157	179.0	179.0	0.608843537414966	0.070166	2.303368	0.5418842461158233	0.6046827468658486	0.6324526234370025	0.6806986606159441	0.7327453024210282	0.8856146157840084	0.5	0.0068859358774662	0.6141219613286742	142	0.656164058684818	16	0.52	1537	0.52	1537	0.8010827193684386	27	0.7677478772846982	19			0	unmatched	0.5	0.5	0.5	0.5	0.0	0.0	Tier 2	Biologically promising but experimentally less ready candidates	Biologically promising candidate requiring additional experimental design or evidence completion.	high_evn_pf_rank;strong_mhc_binding;expressed;dna_supported;rna_allele_supported;acceptable_safety_profile;experimentally_reviewed_candidate	limited_primer_feasibility		1.0	1	0	
chr10:125859826:G:C|LLARRELKL|HLA-B*08:01	chr10:125859826:G:C	DHX32	DHX32	p.P209R	p.P209R	LLARRELKL	HLA-B*08:01	47.309635	354	1.0	260.0	260.0	1.0	0.292495	3.9843	0.7913785799205214	0.5466347635935604	0.8515019357538098	0.5468800984685169	0.6766512331689177	0.982567497403946	1.0	0.0010382917964693	0.7186894182200252	1	0.6896909372065195	4	0.8466666666666667	232	0.9966666666666668	229	0.9040123979945496	5	0.8282087412660487	5	DHX32_P1	DHX32_P1	1	gene_protein_change	0.8833333333333332	1.0	1.0	0.8	0.0	0.1	Tier 1	High-confidence experimentally actionable candidates	High EVN/PF priority with strong biological evidence and actionable primer feasibility. Risk flags: technical_primer_caution.	high_evn_pf_rank;strong_mhc_binding;expressed;dna_supported;rna_allele_supported;primer_feasible;acceptable_safety_profile;experimentally_reviewed_candidate;wet_lab_prioritized_candidate		technical_primer_caution	1.0	1	1	strong positive support
chr5:7886680:G:C|LQIRAIPKK|HLA-A*11:01	chr5:7886680:G:C	MTRR	MTRR	p.E375Q	p.E375Q	LQIRAIPKK	HLA-A*11:01	18.704291	208	0.3195084485407066	32.0	32.0	0.2105263157894736	0.495948	4.144175	0.3536132766389687	0.3922400786631925	0.3261319994603003	0.4973840899785567	0.6454864364654342	0.9785208982346834	1.0	0.0014072884215991	0.594543543938489	337	0.6325421990196216	30	0.8616666666666666	231	0.9616666666666666	232	0.8174796896885361	21	0.7536458825629615	24	MTRR_P1	MTRR_P1	1	gene_protein_change	0.9333333333333332	1.0	1.0	0.5	0.0	0.05	Tier 1	High-confidence experimentally actionable candidates	High EVN/PF priority with strong biological evidence and actionable primer feasibility.	high_evn_pf_rank;strong_mhc_binding;expressed;dna_supported;rna_allele_supported;primer_feasible;acceptable_safety_profile;experimentally_reviewed_candidate;wet_lab_prioritized_candidate			1.0	1	1	low-VAF mixed-peak support
chr15:100900577:G:T|IVCADADLY|HLA-A*29:02	chr15:100900577:G:T	ALDH1A3	ALDH1A3	p.D189Y	p.D189Y	IVCADADLY	HLA-A*29:02	12.246808	174	0.4438775510204081	10.0	10.0	0.238095238095238	0.406733	91.772003	0.4028100766618311	0.3238776991257407	0.2705188633644445	0.5159737208509184	0.8556783385164766	0.9319866303219108	0.5	0.0037340018172377	0.5334624474103535	951	0.6299350646822367	33	0.52	1538	0.52	1538	0.7602971425317671	48	0.7324938353904487	33			0	unmatched	0.5	0.5	0.5	0.5	0.0	0.0	Tier 2	Biologically promising but experimentally less ready candidates	Biologically promising candidate requiring additional experimental design or evidence completion.	high_evn_pf_rank;strong_mhc_binding;expressed;dna_supported;rna_allele_supported;acceptable_safety_profile;experimentally_reviewed_candidate	limited_primer_feasibility		1.0	1	0	
chr16:57046616:C:A|AAAKFLGFRK|HLA-A*11:01	chr16:57046616:C:A	NLRC5	NLRC5	p.Q1077K	p.Q1077K	AAAKFLGFRK	HLA-A*11:01	10.430337	165	0.6021897810218978	18.0	18.0	0.3829787234042553	0.234355	6.41438	0.4848840777161734	0.2984861527982901	0.377484905814786	0.5676534594405079	0.7211517061737872	0.9436786085150572	1.0	0.0041494029075804	0.6321679752602799	57	0.6746652087436387	8	0.8616666666666666	230	0.9616666666666666	231	0.8829801526312507	6	0.8102628595379927	6	NLRC5_P1	NLRC5_P1	1	gene_protein_change	0.9333333333333332	1.0	1.0	0.5	0.0	0.05	Tier 1	High-confidence experimentally actionable candidates	High EVN/PF priority with strong biological evidence and actionable primer feasibility.	high_evn_pf_rank;strong_mhc_binding;expressed;dna_supported;rna_allele_supported;primer_feasible;acceptable_safety_profile;experimentally_reviewed_candidate;wet_lab_prioritized_candidate			1.0	1	1	ALT peak support
chr4:103145956:C:G|KTLQESHDEMK|HLA-A*11:01	chr4:103145956:C:G	CENPE	CENPE	p.R1404T	p.R1404T	KTLQESHDEMK	HLA-A*11:01	8.915718	81	0.2009925558312655	9.0	9.0	0.2368421052631578	0.382928	9.797433	0.1826674678555871	0.2740132449338187	0.2638717039330249	0.5216270734443934	0.7168514482797803	0.9888888888888888	0.5	0.0008888888888888	0.5056919045043171	1503	0.6117074523366605	55	0.52	1542	0.52	1542	0.7319535589465026	87	0.7079943441328028	58			0	unmatched	0.5	0.5	0.5	0.5	0.0	0.0	Tier 2	Biologically promising but experimentally less ready candidates	Biologically promising candidate requiring additional experimental design or evidence completion.	strong_mhc_binding;expressed;dna_supported;rna_allele_supported;acceptable_safety_profile;experimentally_reviewed_candidate	limited_primer_feasibility		1.0	1	0	
chr19:57397066:G:T|TEGRVVFEY|HLA-A*29:02	chr19:57397066:G:T	ZNF548	ZNF548	p.D12Y	p.D12Y	TEGRVVFEY	HLA-A*29:02	5.850882	292	0.4390977443609022	9.0	9.0	0.391304347826087	0.485052	72.532837	0.4580284243931736	0.2103571150494394	0.3411028252144895	0.4994664850572752	0.8294440805849157	0.9869970145379025	0.5	0.0008168159397715	0.5186462547360464	1213	0.6091324343582919	56	0.52	1543	0.52	1543	0.7279494559449835	97	0.704533296725536	66			0	unmatched	0.5	0.5	0.5	0.5	0.0	0.0	Tier 2	Biologically promising but experimentally less ready candidates	Biologically promising candidate requiring additional experimental design or evidence completion.	strong_mhc_binding;expressed;dna_supported;rna_allele_supported;acceptable_safety_profile;experimentally_reviewed_candidate	limited_primer_feasibility		1.0	1	0	
chr6:16306520:G:A|LSAAPFLTK|HLA-A*11:01	chr6:16306520:G:A	ATXN1	ATXN1	p.P753S	p.P753S	LSAAPFLTK	HLA-A*11:01	5.133773	471	0.7136363636363636	35.0	35.0	0.8536585365853658	0.056949	2.761012	0.6641825846455476	0.1913215293049589	0.653194480651354	0.7002623036002324	0.7576124235076527	0.9866984683281412	1.0	0.0009984099169262	0.7145798239816673	2	0.7484200687425339	2	0.8766666666666667	229	0.9766666666666668	230	1.0	1	0.9116457856444866	2	ATXN1_P1	ATXN1_P1	1	gene_protein_change	0.8833333333333332	1.0	1.0	1.0	0.0	0.1	Tier 1	High-confidence experimentally actionable candidates	High EVN/PF priority with strong biological evidence and actionable primer feasibility. Risk flags: technical_primer_caution.	high_evn_pf_rank;strong_mhc_binding;expressed;dna_supported;rna_allele_supported;primer_feasible;acceptable_safety_profile;experimentally_reviewed_candidate;wet_lab_prioritized_candidate		technical_primer_caution	1.0	1	1	ALT peak support
chr10:37957501:C:T|FTKEKWKLL|HLA-B*08:01	chr10:37957501:C:T	ZNF25	ZNF25	p.E21K	p.E21K	FTKEKWKLL	HLA-B*08:01	4.427013	159	0.4718100890207715	24.0	24.0	0.5333333333333333	0.122794	2.009607	0.4082742815164348	0.1702451560336302	0.4699979514083635	0.6282390081210567	0.688354451939374	0.9864615784008308	0.5	0.0008435877466251	0.5353840744666587	929	0.6180563012770887	41	0.52	1540	0.52	1540	0.7418258958824434	71	0.716527747529978	45			0	unmatched	0.5	0.5	0.5	0.5	0.0	0.0	Tier 2	Biologically promising but experimentally less ready candidates	Biologically promising candidate requiring additional experimental design or evidence completion.	high_evn_pf_rank;strong_mhc_binding;expressed;dna_supported;rna_allele_supported;acceptable_safety_profile;experimentally_reviewed_candidate	limited_primer_feasibility		1.0	1	0